In vivo electrophysiological recordings in the mouse auditory brainstem
April 2018 (the exact date will be announced in Jan 2018)
max. 6 participants
Paul-Flechsig-Institute of Brain Research
University of Leipzig
Workshop on CRISPR/Cas9 Genome Editing Methods
- from Basics to New Applications -
November 6-8, 2017, Marburg
Organizer: Prof. Dr. Dominik Oliver
"Co-Detection of mRNA/protein plasticity genes in the ascending auditory pathway"
November 7-11, 2016, Tübingen
Organizer: Prof. Dr. Marlies Knipper
"Viral gene transfer and stereotaxic delivery in the auditory system"
April 4-7, 2016, Heidelberg
Organizers: Christoph Körber (Heidelberg), Tina Pangrisc (Göttingen)
Topics: stereotaxic injections, virus systems, tracer systems and applications, genetic manipulation of the cochlea (theory), hands-on experiments
"Modeling Neurons in the Auditory Brainstem"
June 16-17, 2015 (excursion June 18th), TU Munich, Campus Garching
Organizers: Prof. Dr. Werner Hemmert, Jörg Encke, Marek Rudnicki
With support of Prof. Ray Meddis (Essex) the Bio-inspired Information Processing Group from TU Munich organizes a modeling workshop for auditory neurons. Ray Meddis has offered to introduce his new and open Model of the auditory periphery and auditory brainstem (MAP_BS). The package is based on MATLAB computer code and includes a graphical user interface (GUI) with a number of point and click demonstrations. It includes left- and right inner ear modules, which convert sound signals into action potentials of the auditory nerve. In addition, the package already includes many more auditory brainstem neurons and even efferent feedback from the medial olivo-cochlear (MOC) system. Some outputs are plotted below.
We will also introduce our “cochlea” framework, which includes a collection of inner ear models (e.g. Zilany, Bruce & Carney, 2014). We will show how to connect the framework with the BRIAN simulator and create neuronal networks of auditory brainstem neurons using PYTHON.
"Targeted protein expression and conditional gene deletion in the lower auditory brainstem"
November 30-December 3, 2014, Lausanne
Organizers: Prof. Ralf Schneggenburger, Dr. Olexiy Kochubey
Prof. Carl Petersen, EPFL; Dr. Bernard Schneider, EPFL; Prof. Ralf Schneggenburger, EPFL; Dr. Olexiy Kochubey, EPFL; Prof. Thomas Kuner, Heidelberg; Prof. Hans Gerd Nothwang, Oldenburg; Prof. Lu-Yang Wang, Toronto; Prof. Sam Young, MPI Florida.
The calyx of Held synapse is maybe the largest excitatory synapse in the brain, specialized for ultrafast and reliable information transfer. The calyx of Held serves as a model synapse to study synaptic mechanisms at a large auditory synapses, and molecular steps of synapse development. Nevertheless, up to about 10 years ago, it has been notoriously difficult to perturb calyx of Held function genetically. In recent years, some laboratories including our lab have developed stereotactic approaches in young mice and rats, in order to enable virus-mediated protein overexpression at the calyx synapse. Furthermore, Cre-driver mouse lines have been identified which allow tissue-specific inactivation of floxed alleles. Together, these approaches allow the targeted inactivation of genes, and rescue via virus-mediated protein expression. These approaches have been crucial for studying the role of presynaptic proteins in transmitter release (Han et al. 2011 Neuron; Kochubey & Schneggenburger, 2011 Neuron), and for the identification of signaling pathways and trophic factors for the development of a large auditory synapse (Xiao et al., 2013 Nat. Neuroscience). The in-vivo protein expression approach will also be useful for a range of additional questions, including optogenetic mapping of synaptic connectivity in auditory circuits in-vitro and in-vivo.
- Viral vectors suitable for in-vivo protein overexpression (Dr. Bernard Schneider; Prof. Sam Young).
- Stereotactic approaches and brainstem surgery for newborn mice and rats (Prof. Thomas Kuner; Prof. Sam Young; Dr. Olexiy Kochubey).
- Optogenetic approaches in Cortex and in auditory brainstem (Prof. Carl Petersen; Dr. Olexiy Kochubey).
- Developmental aspects of auditory brainstem synapses and neurons and their positional and genetic identity (Prof. Gerd Nothwang; Prof. Ralf Schneggenburger).
- In-utero electroporation for protein expression in the lower auditory system (Prof. Lu-Yang Wang; Toronto).
"In vivo extracellular recordings in the auditory brainstem (AVCN, MNTB)"
April 8-12, 2013, Leipzig, Dr. Ivan Milenkovic / Prof. Dr. Rudolf Rübsamen
"Whole-mount immunohistochemistry of the mouse organ of Corti"
April 22-26, 2013, Homburg, Prof. Dr. Jutta Engel
A Priority Project of